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jc 1 mitomp detection kit  (Dojindo Labs)
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Dojindo Labs jc 1 mitomp detection kit
( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Jc 1 Mitomp Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/JC-1+MitoMP+Detection+Kit/pmc13626044-424-1-8
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rnascope multiplex fluorescent detection kit version 2  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope multiplex fluorescent detection kit version 2
( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Rnascope Multiplex Fluorescent Detection Kit Version 2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/RNAscope+Multiplex+Fluorescent+Detection+Kit+v2/pmc13580614-347-0-9
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rnascope multiplex fluorescent detection kit version 2 - by Bioz Stars, 2026-10
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deafness gene variant detection array kit  (CapitalBio Corporation)
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CapitalBio Corporation deafness gene variant detection array kit
( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Deafness Gene Variant Detection Array Kit, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/deafness+gene+variant+detection+array+kit/nct06133946-1-20-26
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edu detection kit  (MedChemExpress)
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MedChemExpress edu detection kit
( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Edu Detection Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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edu detection kit - by Bioz Stars, 2026-10
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mda detection kit  (Beyotime)
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( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Mda Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/Lipid+Peroxidation+MDA+Assay+Kit/pmc13633971-445-14-17
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horse anti rabbit mp 7401 immpress igg polymer kit  (Vector Laboratories)
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Vector Laboratories horse anti rabbit mp 7401 immpress igg polymer kit
( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D <t>)</t> <t>JC-1</t> staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
Horse Anti Rabbit Mp 7401 Immpress Igg Polymer Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/ImmPRESS+HRP+Anti-Rabbit+IgG+(Peroxidase)+Polymer+Detection+Kit%2C+made+in+Horse/pmc13634644-94-17-25
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tunel brightred apoptosis detection kit  (Vazyme Biotech Co)
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Vazyme Biotech Co tunel brightred apoptosis detection kit
( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of <t>TUNEL</t> staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.
Tunel Brightred Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v fitc pi apoptosis detection kit  (Elabscience Biotechnology)
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Elabscience Biotechnology annexin v fitc pi apoptosis detection kit
( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of <t>TUNEL</t> staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/Annexin+V-FITC%2FPI+Apoptosis+Kit/pm41893974-85-6-11
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a211  (Vazyme Biotech Co)
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( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of <t>TUNEL</t> staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.
A211, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/detection+kit/Annexin+V-FITC+PI+Apoptosis+Detection+Kit/pm42815326-75-8-9
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immpress hrp antirabbit igg peroxidase polymer detection kit  (Vector Laboratories)
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( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of <t>TUNEL</t> staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.
Immpress Hrp Antirabbit Igg Peroxidase Polymer Detection Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.

Journal: Science Advances

Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination

doi: 10.1126/sciadv.aee4577

Figure Lengend Snippet: ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.

Article Snippet: The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

Techniques: Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Immunofluorescence, Western Blot, Analysis, Activity Assay, Expressing, Immune Cell, Two Tailed Test

( A ) Fluorescence images of ZHDG internalization in MFC cells. ( B ) Quantification of the intracellular ZHDG fluorescence intensity. ( C ) Viability of MFC cells across treatment groups. ( D ) Annexin V–FITC/PI staining (fluorescence and flow cytometry). ( E ) TEM images of ultrastructural damage in MFC cells. ( F ) Relative LDH release. ( G ) Intracellular ATP levels. ( H ) Immunofluorescence analysis of CRT exposure and HMGB1 release. ( I ) Zinpyr-1 staining in different treatment groups. ( J ) Quantification of JC-1 aggregates and monomers. ( K ) JC-1 staining (flow cytometry). ( L ) Extracellular dsDNA release. ( M ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and AIM2. ( N ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( O ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( P ) Western blot analysis of PANoptosis-related proteins in si AIM2 MFC cells treated with ZHDG + NIR. ( Q ) Schematic of ZHDG under NIR photoactivation inducing AIM2-PANoptosome formation and ICD. Data in (B), (C), (F), (G), and (L) are mean ± SD. Statistical analysis for (B), (C), (F), (G), and (L) was performed using a two-tailed unpaired Student’s t test. (Q) was created in BioRender. Fei, G. (2026) https://BioRender.com/wcbo7ma .

Journal: Science Advances

Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination

doi: 10.1126/sciadv.aee4577

Figure Lengend Snippet: ( A ) Fluorescence images of ZHDG internalization in MFC cells. ( B ) Quantification of the intracellular ZHDG fluorescence intensity. ( C ) Viability of MFC cells across treatment groups. ( D ) Annexin V–FITC/PI staining (fluorescence and flow cytometry). ( E ) TEM images of ultrastructural damage in MFC cells. ( F ) Relative LDH release. ( G ) Intracellular ATP levels. ( H ) Immunofluorescence analysis of CRT exposure and HMGB1 release. ( I ) Zinpyr-1 staining in different treatment groups. ( J ) Quantification of JC-1 aggregates and monomers. ( K ) JC-1 staining (flow cytometry). ( L ) Extracellular dsDNA release. ( M ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and AIM2. ( N ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( O ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( P ) Western blot analysis of PANoptosis-related proteins in si AIM2 MFC cells treated with ZHDG + NIR. ( Q ) Schematic of ZHDG under NIR photoactivation inducing AIM2-PANoptosome formation and ICD. Data in (B), (C), (F), (G), and (L) are mean ± SD. Statistical analysis for (B), (C), (F), (G), and (L) was performed using a two-tailed unpaired Student’s t test. (Q) was created in BioRender. Fei, G. (2026) https://BioRender.com/wcbo7ma .

Article Snippet: The JC-1 MitoMP Detection Kit was obtained from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

Techniques: Fluorescence, Staining, Flow Cytometry, Immunofluorescence, Analysis, Western Blot, Two Tailed Test

( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of TUNEL staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.

Journal: eLife

Article Title: Bidirectional redistribution of actomyosin drives epithelial invagination in ascidian siphon tube morphogenesis

doi: 10.7554/eLife.108588

Figure Lengend Snippet: ( A ) Representative images of EdU staining at 14–15 hpf. Orange: EdU-positive nuclei indicating cell proliferation. Blue: DAPI. No EdU signal was detected in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10. ( B ) Representative images of TUNEL staining at 15 hpf. ( B1 ) Positive control: DNase I pretreatment (20 U/ml, 10 min) induced DNA fragmentation. ( B2 ) Negative control: staining performed without terminal deoxynucleotidyl transferase (TdT) enzyme. ( B3 ) Experimental group: no detectable TUNEL signal in the atrial siphon primordium (white dashed outline). Scale bar: 10 μm. n = 10.

Article Snippet: Commercial assay, kit , TUNEL BrightRed Apoptosis Detection Kit , Vazyme , Cat#A113 , .

Techniques: Staining, TUNEL Assay, Positive Control, Negative Control

Journal: eLife

Article Title: Bidirectional redistribution of actomyosin drives epithelial invagination in ascidian siphon tube morphogenesis

doi: 10.7554/eLife.108588

Figure Lengend Snippet:

Article Snippet: Commercial assay, kit , TUNEL BrightRed Apoptosis Detection Kit , Vazyme , Cat#A113 , .

Techniques: Cell-Signaling, Recombinant, Plasmid Preparation, TUNEL Assay, Cloning, Sequencing, Software